12 hours ago
is the basics of fluorescent activated cell sorting or facs and flow cytometry.
facs or what people often call flow cytometry is a technique that allows us to sort cells based on specific parameters. these include cell size or morphology or protein expression. .
a facs sorter has a collection process and can sort your cells and put them into different wells based on their differences (like gfp expression for example) whereas flow cytometry analyzers don’t collect the cells - they simply analyze them as the name suggests. .
this technique is used by a wide range of people for varying experiments. some people want to study the morphology of their cells, others want to see if a protein is present in the cell, some want to see if their cells have died, and like i mentioned earlier, counting and sorting are often needed to sort out populations of cell types from a mixture. like pulling out the various ingredients from a fruit salad. .
swipe to see a schematic! depending on what you wish to measure or sort, the user changes the parameters of the analysis or sort to suit their needs. in the lab i often want to measure the expression of receptors on the surface of my cells, so we use an antibody conjugated to a fluorophore and use a specific laser to see what population of our cells express the receptor and at what level! .
do you use facs or flow cytometry?